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旨在研究补饲瘤胃源活毕赤酵母(Pichia kudriavzevii,YPK)菌液对奶水牛瘤胃发酵和饲料营养物质降解特性的影响。选取6头体重相近、健康状况良好且装有永久性瘤胃瘘管的摩拉奶水牛为试验动物，随机分为2组，每组3头，分别为对照组(饲喂基础日粮)和处理组(饲喂基础日粮+每头4×1011 CFU/d YPK菌液);测定奶水牛瘤胃发酵参数，采用尼龙袋法评定奶水牛瘤胃内不同发酵时间(2、4、8、12、24、48、72 h)饲料的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的降解特性。结果：与对照组相比，饲喂YPK极显著提高了瘤胃内乙酸(P<0.001)、丙酸(P=0.002)和总挥发性脂肪酸(P<0.001)浓度；饲喂YPK显著提高了DM(P<0.001)、CP(P=0.044)、NDF(P<0.001)、ADF(P<0.001)的瘤胃有效降解率。结论：日粮中添加YPK显著提高了奶水牛瘤胃乙酸、丙酸和总挥发性脂肪酸浓度，提高了饲料营养物质瘤胃降解率，YPK具有开发成反刍动物功能益生菌的潜力。
Abstract：This study aimed to investigate the effects of rumen derived live Pichia kudriavzevii(YPK) culture on rumen fermentation and feed nutrient degradation characteristics in dairy buffalo. Six Murrah buffaloes with permanent rumen fistula were used in this study. The six buffaloes were randomly assigned into two groups; the control group was fed with the basal diet, while the treatment group was fed with the basal diet supplemented with YPK bacterial solution at a dose of 4 × 1011 CFU/d per head. Then, the rumen volatile fatty acids profile in the buffaloes was measured. A nylon bag method was used to evaluate the degradation characteristics of feed dry matter(DM), crude protein(CP), neutral detergent fiber(NDF), and acidic detergent fiber(ADF) in the rumen of the animals at different time points(2, 4, 8, 12, 24, 48, 72 hours post the beginning of the test). The results showed that YPK significantly increased the concentration of acetic acid(P<0.001), propionic acid(P=0.002), and total volatile fatty acids(P<0.001) in the rumen of the buffaloes; YPK significantly increased their rumen degradation rates of feed DM( P<0. 001),CP( P = 0. 044),NDF( P< 0. 001),and ADF( P< 0. 001). In conclusion,YPK significantly increased the rumen fermentation and the degradation rate of feed nutrients in dairy buffaloes. YPK has the potential to be a functional probiotic for ruminants.
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基本信息:
DOI：
中图分类号:S823.5
引用信息:
[1]肖芬,毛家真,向小娥等.补饲瘤胃源活毕赤酵母菌液对奶水牛瘤胃发酵和营养物质降解率的影响[J].畜牧与兽医,2025,57(08):22-27.
基金信息:
广西科技重大专项(桂科AA22068099)