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旨在探究杜撒×大长撒F1代猪磷酸烯醇式丙酮酸羧激酶1(PCK1)基因第二外显子的多态性及其对生长性状的影响。以326头杜撒×大长撒F1代猪为研究对象，对其PCK1基因第二外显子区域进行PCR扩增和一代测序检测单核苷酸多态性(SNP)位点，在此基础上，通过最小二乘模型将检测到的各SNP位点不同基因型及其单倍型组合与5个生长性状进行关联分析。结果:在杜撒×大长撒F1代猪PCK1基因第二外显子区域检测到5个SNP位点，其中g. 57930894A>G、g. 57930906G>A和g. 57930912G>A位于5'-UTR区域，g. 57931021C>T和g. 57931090C>T为同义突变。5个位点分别以AG、GG、GG、CC、CC基因型和A、G、G、C、C等位基因频率最高;除g. 57930894A>G位点外，其余位点均符合哈迪-温伯格平衡(P<0.05)。关联研究结果显示，g. 57930894A>G和g. 57930906G>A位点GG基因型与同位点的其他基因型相比能显著降低达100 kg体重日龄(P<0.01); g. 57930906G>A位点GG基因型个体达60 kg体重日龄也显著低于同位点其他基因型(P<0.01)，且该基因型个体30～60 kg体重阶段的日增重显著高于AA和GA基因型个体(P<0.05); g. 57931090C>T位点CT基因型30～100 kg体重阶段的日增重显著高于CC基因型个体(P<0.05)。单倍型组合与生长性状关联结果显示，AGGCC/GGGTC组合单倍型个体达60 kg体重日龄和达100 kg体重的日龄最长，而以GGGCC/GGGTC和GGGCC/GGGCC组合的日龄最短; 30～60 kg和30～100 kg体重阶段的日增重均以AGGCT/GGGCC组合最高。具有GGGCC单倍型个体的各生长性状均处于较优水平。研究结果初步揭示了杜撒×大长撒F1代猪PCK1基因第二外显子存在与生长性状显著关联的SNP位点，GGGCC单倍型可作为影响生长性状潜在的遗传标记进行后续研究。
Abstract：This study was to explore the polymorphism of exon 2 of phosphoenolpyruvate carboxykinase 1( PCK1) gene in F1 generation of DS×YLS pigs and its effect on growth traits. Single nucleotide polymorphisms( SNP) of exon 2 of the PCK1 gene in 326 F1 generation of DS×YLS pigs were detected by PCR product directed sequencing; and then,the least square model was used to analyze the effects of different genotypes and haplotype combinations of each SNP locus on five growth traits. Five SNPs were detected in exon 2 of the PCK1 gene in F1 generation of the DS×YLS pigs. Among them,g. 57930894A,g. 57930906G>A and g. 57930912G>A were located in the 5'-UTR region; and g. 57931021C>T and g. 57931090C>T were synonymous mutations. The genotypes of AG,GG,GG,CC,CC and A,G,G,C,and C alleles had the highest frequency,respectively. Except the g. 57930894A>G site,all the other sites were in accordance with the Hardy-Weinberg equilibrium( P<0. 05). Associated findings showed that the g. 57930894A>G locus and the g. 57930906G>A locus GG genotype significantly reduced the days to 100 kg body weight( P<0. 01). At locus g. 57930906G>A,individuals of the GG genotype were significantly lower than the other genotypes at the same locus in days to 60 kg body weight( P<0. 01),and individuals of the GG genotype had significantly higher30-60 kg average daily gain( ADG) than the individuals of the AA genotype and the GA genotype( P < 0. 05). The CT genotype at the g. 57931090C>T locus had significantly higher 30-100 kg ADG than the CC genotype( P<0. 05). The results of the association of haplotype combinations with growth traits showed that both the days to 60 kg body weight and the days to 100 kg body weight were the longest in the AGGCC/GGGTC combination,but they were the shortest in the GGGCC/GGGTC and GGGCC/GGGCC combinations,respectively. 30-60 kg ADG and 30-100 kg ADG were the highest in the AGGCT/GGGCC combination. Individuals with the GGGCC haplotype were found to be excellent in all growth traits. The results of this study preliminarily revealed that there were multiple SNP loci significantly associated with growth traits in exon 2 of the PCK1 gene in F1 generation of DS×YLS pigs,and that the GGGCC haplotype could be followed up as a potential genetic marker affecting growth traits.
[1]YU S, MENG S, XIANG M, et al. Phosphoenolpyruvate carboxykinase in cell metabolism:roles and mechanisms beyond gluconeogenesis[J]. Mol Metab,2021,53:101257.
[2]HANSON R W,GARBER A J. Phosphoenolpyruvate carboxykinase.I. Its role in gluconeogenesis[J]. Am J Clin Nutr, 1972, 25(10):1010-1021.
[3]YANG J,KALHAN S C,HANSON R W. What is the metabolic role of phosphoenolpyruvate carboxykinase[J]. J Biol Chem,2009,284(40):27025-27029.
[4]CHEN L,ZENG T,LI G Q,et al. PCK1 expression is correlated with the plasma glucose level in the duck[J]. Anim Genet,2017,48(3):358-361.
[5]GóMEZ-VALADéS A G, MéNDEZ-LUCAS A, VIDALALABRóA,et al. Pck1 gene silencing in the liver improves glycemia control, insulin sensitivity, and dyslipidemia in db/db mice[J]. Diabetes,2008,57(8):2199-2210.
[6]VIEIRA P,CAMERON J,RAHIKKALA E,et al. Novel homozygous PCK1 mutation causing cytosolic phosphoenolpyruvate carboxykinase deficiency presenting as childhood hypoglycemia,an abnormal pattern of urine metabolites and liver dysfunction[J]. Mol Genet Metab,2017,120(4):337-341.
[7]HAKIMI P,YANG J,CASADESUS G,et al. Overexpression of the cytosolic form of phosphoenolpyruvate carboxykinase(GTP)in skeletal muscle repatterns energy metabolism in the mouse[J]. J Biol Chem,2007,282(45):32844-32855.
[8]CAO H,VAN DER VEER E,BAN M R,et al. Promoter polymorphism in PCK1(phosphoenolpyruvate carboxykinase gene)associated with type 2 diabetes mellitus[J]. J Clin Endocrinol Metab,2004,89(2):898-903.
[9]OISHI K,SIEGEL C,CORK E E,et al. Novel missense variants in PCK1 gene cause cytosolic PEPCK deficiency with growth failure from inadequate caloric intake[J]. J Hum Genet,2021,66(3):321-325.
[10]MILLWARD C A, DESANTIS D, HSIEH C W,et al. Phosphoenolpyruvate carboxykinase(Pck1)helps regulate the triglyceride/fatty acid cycle and development of insulin resistance in mice[J]. J Lipid Res,2010,51(6):1452-1463.
[11]FRANCKHAUSER S,MU?OZ S,PUJOL A,et al. Increased fatty acid re-esterification by PEPCK overexpression in adipose tissue leads to obesity without insulin resistance[J]. Diabetes,2002,51(3):624-630.
[12]FRANCKHAUSER S,MU?OZ S,ELIAS I,et al. Adipose overexpression of phosphoenolpyruvate carboxykinase leads to high susceptibility to diet-induced insulin resistance and obesity[J].Diabetes,2006,55(2):273-280.
[13]BEALE E G,HARVEY B J,FOREST C. PCK1 and PCK2 as candidate diabetes and obesity genes[J]. Cell Biochem Biophys,2007,48(2/3):89-95.
[14]JI J,ZHOU L,GUO Y,et al. Genome-wide association study identifies 22 new loci for body dimension and body weight traits in a White Duroc×Erhualian F2intercross population[J]. Asian-Australas J Anim Sci,2017,30(8):1066-1073.
[15]JIANG Y,TANG S,WANG C,et al. A genome-wide association study of growth and fatness traits in two pig populations with different genetic backgrounds[J]. J Anim Sci,2018,96(3):806-816.
[16]WANG K,WU P X,WANG S J,et al. Epigenome-wide DNA methylation analysis reveals differentially methylation patterns in skeletal muscle between Chinese Chenghua and Qingyu pigs[J]. J Integr Agric,2022,21(6):1731-1739.
[17]NIE J,ZHANG B,MA L,et al. Comparative transcriptomic and proteomic analyses provide insights into the key genes involved in muscle growth in the large Diqing Tibetan pig[J]. Can J Anim Sci,2023,103(4):373-387.
[18]朱叶，张艳，王孝义，等．基于转录组测序发掘大河猪背脂沉积的关键基因[J]．云南农业大学学报(自然科学)，2024,39(5):23-30.
[19]BIAN L,DI Z,XU M,et al. Transcriptome analysis reveals the early development in subcutaneous adipose tissue of Laiwu piglets[J]. Animals,2024,14(20):2955.
[20]刘华涛，刘怡冰，石咏，等．鉴别参与调控长白猪脂肪沉积的重要基因和lncRNA[J]．中国畜牧杂志，2021,57(S1):143-151.
[21]WANG Y,ZHANG W,WU X,et al. Transcriptomic comparison of liver tissue between Anqing six-end-white pigs and Yorkshire pigs based on RNA sequencing[J]. Genome,2020,63(4):203-214.
[22]XING K,WANG K,AO H,et al. Comparative adipose transcriptome analysis digs out genes related to fat deposition in two pig breeds[J]. Sci Rep,2019,9(1):12925.
[23]TAN Z,WANG Y,YANG T,et al. Differentially expressed genes in the caecal and colonic mucosa of Landrace finishing pigs with high and low food conversion ratios[J]. Sci Rep, 2017, 7(1):14886.
[24]LATORRE P,BURGOS C,HIDALGO J,et al. c. A2456C-substitution in Pck1 changes the enzyme kinetic and functional properties modifying fat distribution in pigs[J]. Sci Rep,2016,6:19617.
[25]聂光伟．猪LTBP2、DLST、TGFB3、PCK1和MAP2K6基因SNPs鉴定及其与重要经济性状的关联研究[D]．武汉:华中农业大学，2018.
[26]展西振，刘军，彭雅鑫，等．猪PCK1基因SNPs检测及其与生长育肥性状的关联分析[J]．中国畜牧杂志，2023,59(11):106-111,118.
[27]鲁绍雄，连林生．SAS统计分析系统在畜牧科学中的应用[M]．昆明:云南科技出版社，2003.
[28]林嘉颖，袁源，刘新光．磷酸烯醇式丙酮酸羧激酶1(PCK1)的研究进展[J]．生命的化学，2019,39(4):637-642.
[29]CHAKRAVARTY K,CASSUTO H,RESHEF L,et al. Factors that control the tissue-specific transcription of the gene for phosphoenolpyruvate carboxykinase-C[J]. Crit Rev Biochem Mol Biol,2005,40(3):129-154.
[30]鲁绍雄，李明丽，严达伟，等．云南地方猪种质特性及其保种与多样化利用[J]．云南农业大学学报(自然科学)，2020,35(6):1096-1105.
[31]连林生，鲁绍雄，严达伟，等．滇撒猪配套系选育[C]. 2006中国猪业发展大会，海口，2006.
基本信息:
中图分类号:S828
引用信息:
[1]赖金花,王孝义,朱怡轩,等.杜撒×大长撒F_1代猪PCK1基因多态性及其与生长性状的关联研究[J].畜牧与兽医,2026,58(01):1-7.
基金信息:
云南省生猪产业技术体系建设项目(2023KJX0016); 云南省产业技术领军人才专项(YNWR-CYJS-2018-056)