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为了解目前河南地区猪流行性腹泻病毒(PEDV)流行毒株的基因型和流行情况，于2023年11月到2025年2月期间，在河南周口、南阳、郑州、许昌、平顶山、濮阳、鹤壁等18个地区的23个中小型猪场共收集62份发生仔猪腹泻的临床肠道、粪便样本，对样本进行核酸提取后，采用RT-PCR方法检测样本的PEDV N基因。对PEDV阳性样本的S基因全长进行扩增和测序，并将所得PEDV S基因全长序列进行构建遗传进化树、核苷酸、氨基酸同源性分析。结果:62份样本中PEDV阳性样本为38份，阳性率为61.29%; 38份阳性样本中有19份扩增出S基因全长;同源性分析显示PEDV S基因全长的核苷酸同源性为93.4%～100%，氨基酸同源性为80.9%～87.7%，与GⅠ基因型经典毒株CV777的核苷酸同源性为89.7%～93.3%，氨基酸同源性为73.1%～75.9%;与PEDV的近几年流行GⅡ亚型毒株CH-He N-ZLH-2022核苷酸同源性为94.8%～99.2%，氨基酸同源性为83.0%～86.5%;遗传进化树结果显示目前河南地区PEDV流行情况以GⅡ亚型为主，其中15株为GⅡa亚型、4株为GⅡc亚型;另外，根据搜集样本的时间来看，在近期搜集的样本中属于GⅡc亚型的比例呈现上升趋势，说明GⅡc亚型毒株呈现成为优势流行毒株的趋势。本试验通过对现阶段河南省PEDV S基因流行变异情况进行了分析，为未来河南省PED的疫情防控和疫苗研发提供参考依据。
Abstract：To understand the genotypes and epidemic status of currently circulating PEDV strains in Henan Province,China,a total of 62 clinical intestinal and fecal samples from diarrheic piglets were collected in the period between November 2023 and February 2025. These samples were collected on 23 small to medium-sized pig farms across 18 regions in Henan,including Zhoukou,Nanyang,Zhengzhou,Xuchang,Pingdingshan,Puyang,Hebi and so on. Following nucleic acid extraction,the samples were screened for PEDV using RT-PCR targeting the N gene. The full-length S gene of the PEDV-positive samples was amplified,sequenced,and subjected to phylogenetic tree construction,as well as nucleotide and amino acid homology analyses. The results showed that 38 out of the 62 samples( 61. 29%) were PEDV-positive. Among these,the full-length S gene was successfully amplified from 19 samples. The homology analysis showed that the nucleotide sequence identity of the full-length S gene among the strains ranged from 93. 4% to 100%,and the amino acid identity ranged from80. 9% to 87. 7%. Compared with the classical GⅠ genotype strain CV777,the nucleotide similarity was 89. 7%-93. 3%,and the amino acid identity was 73. 1%-75. 9%. In contrast,the strains showed higher nucleotide identity( 94. 8%-99. 2%) and amino acid identity( 83. 0%-86. 5%) with the recently prevalent GⅡ subtype strain CH-He N-ZLH-2022. The phylogenetic analysis indicated that the predominant circulating strains in the Henan area belonged to the GⅡ subtype,specifically with 15 strains classified as GIIa and 4 as GⅡc.Furthermore,the temporal analysis of the collected samples exhibited an increasing proportion of the GⅡc subtype in recent collections,suggesting a potential trend for GⅡc strains to become the predominant epidemic variant. This study provided an analysis of the current prevalence and variation of the PEDV S gene in Henan Province,offering valuable insights for future PED prevention,control strategies,and vaccine development in the region.
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基本信息:
中图分类号:S858.28
引用信息:
[1]范金鹏,石蒙蒙,李永涛,等.2023—2025年河南地区猪流行性腹泻病毒分子流行病学调查[J].畜牧与兽医,2026,58(05):99-108.
基金信息:
河南省自然科学基金青年科学基金项目(252300423606); 国家自然科学基金青年科学基金(C类)项目(32503038); 河南省博士后科研启动基金资助项目(HN2022124); 校企合作资助项目(30803350)
2026-05-07
2026-05-07